Journal: Plant Physiology

Article Title: Light Sheet Microscopy Imaging of Light Absorption and Photosynthesis Distribution in Plant Tissue ¹

doi: 10.1104/pp.17.00820

Figure Lengend Snippet: Photosynthetic ETR from PSII versus photon absorption curve parameters and the fractional photon absorption (Abs; in percentage of total absorption) calculated for the upper cortex, medulla, and lower cortex under blue (425–475 nm), green (525–575 nm), red (615–665 nm), and white (400–700 nm) irradiance applied perpendicular to one side of the thallus surface of F. vesiculosus Slopes on the subsaturated part of the ETR versus light curve, maximum ETR values (ETR max ), and the light acclimation index Ek were estimated from curve fitting of the ETR versus photon absorption curves with an exponential function ( Webb et al., 1974 ) using a nonlinear Levenberg-Marquardt fitting algorithm. The RGB values were calculated as average curves of blue, green, and red. Pairwise superscript letters indicate statistically significant differences (one-way ANOVA, P < 0.01; n = 5 for white and n = 4 for RGB ).

Article Snippet: The light saturation coefficient (i.e. the photon scalar irradiance at the onset of light saturation of photosynthesis, E k ) was calculated as , where ETR max is the maximum activity and α is the initial slope of the ETR versus photon absorption curve; both parameters were obtained by curve fitting of ETR measurements using an exponential function ( Webb et al., 1974 ) by means of a nonlinear Levenberg-Marquardt fitting algorithm (OriginPro 2015; OriginLab).

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